Temperature-sensitive transformation by Rous sarcoma virus and temperature-sensitive protein kinase activity
نویسندگان
چکیده
منابع مشابه
Transformation by a temperature sensitive mutant of Rous sarcoma virus in the absence of serum.
Cultures of chicken embryo fibroblasts infected with the temperature-sensitive transformation mutant of Rous sarcoma virus, tsLA24PR-A, were arrested between mitosis and S phase by exposure to serum-free medium at the non-permissive temperature (41degree C) for 2 days. On shifting to the permissive temperature (35degree C) the cells assumed a transformed morphology and increased uptake of [2min...
متن کاملTransformation of chick-embryo fibroblasts by wild-type and temperature-sensitive Rous sarcoma virus alters adenylate cyclase activity.
The activity of the enzyme adenylate cyclase, a component of the plasma membrane, has been determined in chick-embryo fibroblasts and in cells transformed by either Bryan high-titer strain of Rous sarcoma virus (RSV-BH) or a temperature-sensitive mutant of this virus (RSV-BH-Ta). Adenylate cyclase activity is reduced in cells transformed by the wild-type virus and also by the temperature-sensit...
متن کاملCooperative transformation studies with temperature-sensitive mutants of Rous sarcoma virus.
Stocks of Rous sarcoma virus Bryan strain were mutagenized using a bromodeoxyuridine treatment immediately after infection. Thirty temperature-sensitive (ts) mutants defective in transformation (td) were isolated by a replica plating technique. Twenty of these mutants were preliminarily characterized and found to be defective in late functions related to transformation. These mutants were used ...
متن کاملTemperature-dependent alterations in sugar transport in cells infected by a temperature-sensitive mutant of Rous sarcoma virus.
Cells transformed by Rous sarcoma virus take up 2-deoxyglucose at a faster rate than uninfected cells, under conditions where transformed and nontransformed cells grow at the same rate. In cells infected by a temperature-sensitive mutant, the stimulation of 2-deoxyglucose uptake is temperature dependent: the increase is observed at the permissive (36 degrees C), but not at the nonpermissive (41...
متن کاملModulation of fibronectin gene activity in chick embryo fibroblasts transformed by a temperature-sensitive strain (ts68) of Rous sarcoma virus.
Transcriptional regulation of the fibronectin gene is a major mechanism for lowering steady-state levels of fibronectin mRNA in chick embryo fibroblasts (CEF) transformed by Rous sarcoma virus (RSV) (1). In the present study, we have measured the change of transcriptional activity of the fibronectin gene in CEF transformed by a temperature-sensitive strain of RSV (ts68). Ts68-CEF maintained at ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1980
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.33.1.220-229.1980